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Rev. biol. trop ; 54(4): 1135-1141, dic. 2006. graf
Article in Spanish | LILACS | ID: lil-492169

ABSTRACT

A survey was conducted in 30 fields located at three different altitudes in Cartago, Costa Rica's main potato producing area. Twenty plants were sampled per farm, for a total of 600 samples with 200 samples per altitude. ELISA was used with commercial reagents to independently test for PVX, PVY, PVM, PVA, PVS, PLRV, PMTV, PAMV, PVV, PVT, APLV, APMoV and TRSV. The presence of the following viruses was determined: PVX (77 %), PAMV (62 %), PLRV (42 %), TRSV (42 %), PVT (39 %), PVV (37 %), PMTV (31%), PVY (30 %), PVS (19 %), PVM (13 %), PVA (8 %), and APMoV (8%). APLV was not detected in any sample. This is the first report in Costa Rica of the presence of the viruses PMTV, PAMV, PVV, PVT and APMoV. A high viral incidence in the tuber seed production area as well as a high rate of mixed infections is reported.


En Cartago, la zona productora de papa más importante de Costa Rica, se realizó un muestreo en 30 fincas ubicadas a tres altitudes. Se recolectaron 20 plantas por finca y 200 muestras por altitud. Todas las muestras se analizaron independientemente mediante ELISA, para PVX, PVY, PVM, PVA, PVS, PLRV, PMTV, PAMV, PVV, PVT, APLV, APMoV y TRSV, utilizando reactivos comerciales. Se identificó la presencia de PVX (77 %), PAMV (62 %), PLRV (42 %), TRSV (42 %), PVT (39 %), PVV (37 %), PMTV (31 %), PVY (30 %), PVS (19 %), PVM (13 %), PVA (8 %), y APMoV (8 %). No se detectó APLV en ninguna de las muestras analizadas. Se informan por primera vez la presencia en Costa Rica de los virus PMTV, PAMV, PVV, PVT y APMoV. Se informa la alta incidencia viral en la zona dedicada a la producción de tubérculos como semilla y la alta tasa de infecciones mixtas.


Subject(s)
Altitude , Plant Diseases/virology , Solanum tuberosum/virology , Plant Viruses/classification , Enzyme-Linked Immunosorbent Assay , Costa Rica/epidemiology , Plant Diseases/statistics & numerical data , Incidence , Prevalence , Plant Viruses/isolation & purification
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